Abstract
The aim of the present work was to explore the composition, molecular weight and monosaccharide composition of ,Flammulina velutipes polysaccharides by DEAE Cellulose-52, High Performance Gel Filtration Chromatography (HPGFC) and HPLC with PMP derivative with the FVP isolated and purified from Flammulina velutipes. The results showed that Flammulina velutipes polysaccharides were composed of three kinds of polysaccharides; the molecular weight of FVP was composed of 4191338, 372779, 19002; the molecular weight of 372779, 19002, one of the biggest average molecular weight polysaccharides is neutral polysaccharide, the rest of the two kinds of polysaccharide composition of less average molecular weight is acidic polysaccharides and the proportion of two kind of acidic polysaccharides significantly greater than neutral polysaccharide, 44.31 and 37.76% respectively, only 17.93% of neutral polysaccharide. FVP was composed of Glc, Man, Gal, Xyl, Fuc; the highest content of Glc, followed by Gal and Man, Xyl and Fuc content is relatively low. The molar ratio was relatively 13.05:2.75:3.16:1.48:1.00. In conclusion, FVP contained the more Glc, the less Fuc and a small amount of galacturonic acid and glucuronic acid, which showed FVP may contain acidic polysaccharides.
Highlights
Polysaccharide is one of the important forms of naturally occurring sugar. It usually consists of more than 10 monosaccharide bases which are connected by glycosidic bond (Abdel-Akher et al, 1952; Agrawal, 1992; Pang et al, 2007), including polysaccharides (Ikekawa et al, 1982), storage structure and biological activity of polysaccharide (Mallavadhani et al, 2006; Pang et al, 2007)
Because bioactive polysaccharide has much important biological activity, it has been applied in many areas such as functional food, biological medicine, biological materials (Wasser, 2002; Zhang et al, 2007)
Bioactive polysaccharide is usually composed of several hundred to several thousand monosaccharides polymerization, whose nature is completely different from monosaccharides and biological activity of polysaccharides are related to its monosaccharide composition, molecular weight and structure (Yan et al, 2004)
Summary
Polysaccharide is one of the important forms of naturally occurring sugar It usually consists of more than 10 monosaccharide bases which are connected by glycosidic bond (Abdel-Akher et al, 1952; Agrawal, 1992; Pang et al, 2007), including polysaccharides (Ikekawa et al, 1982), storage structure and biological activity of polysaccharide (Mallavadhani et al, 2006; Pang et al, 2007). Bioactive polysaccharide is usually composed of several hundred to several thousand monosaccharides polymerization, whose nature is completely different from monosaccharides and biological activity of polysaccharides are related to its monosaccharide composition, molecular weight and structure (Yan et al, 2004). Flammulina velutipes Polysaccharides (FVP) is the most abundant biological active compounds content of Flammulina velutipes, has the good immunity, antitumor, protecting liver and enhance memory (Leung et al, 1997; Zheng et al, 2005; Peng et al, 2005).
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