Abstract
Fluorescent probes are powerful tools for real-time tracking of lysosomal movements and spatial distribution, which appears to be essential for understanding the biology of lysosomes. However, the commercially used lysosomal trackers exhibited some limitations, such as narrow Stokes shifts, poor photostability, and green to red instead of near-infrared (NIR) emission. In this study, we have developed a fluorescent probe by incorporating the triphenylamine fragment into the dicyanoisophorone fluorophore. This probe displayed impressive NIR emission (centered at 755 nm) and remarkable Stokes shift (235 nm), which was then demonstrated to be insensitive to lysosomal pH as well as viscosity, and expressed long-term lysosomal tracking ability with little cytotoxicity in live cells. Altogether, this study provided an ideal NIR fluorescent probe for lysosomal tracking.
Published Version
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