Discordance between cortical and trabecular bone phenotype highlights the role of local versus circulating IGF-1 in the SOCS2 null mouse

Abstract

Event Abstract Back to Event Discordance between cortical and trabecular bone phenotype highlights the role of local versus circulating IGF-1 in the SOCS2 null mouse R Dobie1*, C Huesa1, RJ Van'T Hof2, VE Macrae1, SF Ahmed3 and C Farquharson1 1 University of Edinburgh, Roslin Institute & R(D)SVS, United Kingdom 2 University of Edinburgh, Centre for Molecular Medicine, United Kingdom 3 University of Glasgow, Bone and Endocrine Research Group, United Kingdom Suppressor of Cytokine Signalling 2 (SOCS2) tightly regulates postnatal growth through its actions on the GH/IGF-1 pathway. SOCS2 knockout mice do not have raised circulating IGF-1 and are characterised by enhanced body size and bone length. However the trabecular architecture and cortical geometry of the bones have yet to be fully investigated. Further to this, the bone mechanical properties remain undefined. In order to better appreciate the role of SOCS2 in juvenile and adult male and female mice, we have conducted an in depth analysis of the bone phenotype. {BR}Micro-CT was carried out on the tibia of wild-type (WT) and SOCS2 knockout (KO) mice, at 6-weeks (juvenile) and 4-months (adult) of age. Analysis enabled detailed comparisons to be made between the trabecular and cortical macroarchitecture, and allowed for the calculation of the mineral density of the bone tissue. 3-point-bending was also completed to investigate the mechanical properties. {BR}A significant increase in body weight was observed in all KO mice (P<0.05). All KO mice had increased trabecular BV/TV compared to their age and sex matched WT controls which is a likely consequence of increased trabecular thickness (P<0.05). All KO mice had increased tibial periosteal surface area (P<0.05), however the cortex was thicker only in juvenile and adult KO male mice. The thicker cortex of the male KO mice tibia was associated with increased work to failure and maximum load. At 6 weeks of age there was no difference in the mineral density of the bone tissue in either gender, however at 4 months both male and female KO mice had a significant decrease of mineral density in the cortical bone (P<0.05).{BR}The increased bone size and mass are consistent with the known anabolic effects of local, skeletal, IGF-1 and these effects are age and sex specific. The fall in mineral density of cortical bone reflects the importance of circulating IGF-1 on cortical bone development. The SOCS2 KO mouse represents a valid model for studying the effects of GH and IGF-1 on bone. Keywords: Bones, Bone Research Conference: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society, Cambridge, United Kingdom, 27 Jun - 29 Jun, 2011. Presentation Type: Oral Topic: Abstracts Citation: Dobie R, Huesa C, Van'T Hof R, Macrae V, Ahmed S and Farquharson C (2011). Discordance between cortical and trabecular bone phenotype highlights the role of local versus circulating IGF-1 in the SOCS2 null mouse. Front. Endocrinol. Conference Abstract: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society. doi: 10.3389/conf.fendo.2011.02.00015 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2011; Published Online: 30 Sep 2011. * Correspondence: Mr. R Dobie, University of Edinburgh, Roslin Institute & R(D)SVS, United Kingdom, ross.dobie@roslin.ed.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers R Dobie C Huesa RJ Van'T Hof VE Macrae SF Ahmed C Farquharson Google R Dobie C Huesa RJ Van'T Hof VE Macrae SF Ahmed C Farquharson Google Scholar R Dobie C Huesa RJ Van'T Hof VE Macrae SF Ahmed C Farquharson PubMed R Dobie C Huesa RJ Van'T Hof VE Macrae SF Ahmed C Farquharson Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.