Abstract
Two large, hydrophobic polypeptides termed PSI-A and PSI-B of Photosystem I are thought, as a heterodimer, to harbor all redox centers and pigments required for primary charge separation. The gene psaB coding for PSI-B was cloned from Anabaena variabilis , a segment replaced by a drug-resistance cassette, and returned to Anabaena on a suicide plasmid. Double recombinants (mutants) grew only chemoheterotrophically, had a reduced chlorophyll content, and lacked Photosystem I.
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