Abstract
The impregnation of the edges of plates before carrying out forced-flow thin-layer chromatography (TLC) as overpressured TLC is time consuming. This paper reports the optimisation of analytical parameters and the direct use of a new separation medium, Empore TLC sheets, for forced-flow TLC. The pressure of the water pump ( P) and the flow-rate of the eluent ( F) were studied and optimum values of P = 2–4 bar and F = 0.4–0.6 ml/min were determined. The separation of a mixture of dyes and a bergamot oil was investigated and the results demonstrated that the direct utilisation of Empore TLC sheets in forced-flow TLC gave performances similar to pre-impregnated high-performance TLC plates.
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