Abstract
Embryogenic callus from four asparagus genotypes (JG8, MD10, MD22, and 86SOM1) was initiated from micropropagated spears placed on semisolid LS medium containing 5 μM 2,4-D or 50 μM NAA, concomitantly. After three subcultures such cells were used to initiate suspensions in liquid medium of the same composition. The eight sets of suspensions were used as sources of protoplasts at two months of age and again at five months. Protoplasts were immobilized at 105/ml density in MS medium with 0.6% agarose and overlaid with liquid KM medium containing the auxin of the corresponding donor suspension or no hormones. Plating efficiencies were recorded at 14 days and ranged from 0% to 40% depending on the genotype, suspension medium, and inclusion or exclusion of hormones in the protoplast plating medium. All four genotypes were capable of forming somatic embryos directly from protoplasts; however, conversion was greatest from MD10 and MD22 derived cultures and occurred as rapidly as six weeks after initial protoplast culture.
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