양파의 성숙배 배양을 통한 체세포배발생 캘러스 유기 및 식물체 재분화

Abstract

To obtain regeneration system of onion, we analyzed the effects of 2,4-D and BA concentration on the embryogenic callus induction from mature embryos. The highest embryogenic callus induction ratio was shown on MS medium (Murashie and Skoog 1962) containing picloram after mature embryos were placed on medium. When induced callus were cultured on half strength of MS medium containing Kinetin, the highest shoot formation ratio was observed on MS medium containing 2,4-D and BA. Embryogenic callus were cultured in MS liquid medium containing of 2,4-D and BA. The suspension cultured cell clumps could be mass propagated. Embryogenic callus were friable, but non-embryogenic callus included a lot of moisture, hence the identification between embryogenic and non-embryogenic callus as easily achieved. When embryogenic callus as cultured on half strength of MS medium containing Kinetin, shoots were induced. The whole plantlet was obtained on rooting medium containing of NAA.