Abstract
This study reports a simple micropropagation protocol and thereby rapid multiplication of the useful medicinal plant- Scoparia dulcis L. Single node explants were inoculated on basal MS medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA) for direct plant regeneration. Maximum numbers of shoot (~22) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and rooted on half strength MS medium supplemented with 0.5 mg/l of IBA alone for three weeks. Simultaneous regeneration of shoots and roots and in vitro flowering were achieved from the nodal explants on MS medium supplemented with 0.5 mg/l KN and 2.0 mg/l IAA. Well-developed complete plantlets were transferred on to specially made plastic cup containing soil rite. Acclimatized plantlets were successfully grown in garden soil.
Highlights
(w/v) sucrose, supplemented with different concentrations constraints
Shoots were separated and rooted on half strength MS Materials and methods medium supplemented with 0.5 mg/l of indole-3butyric acid (IBA) alone for Explants were collected from moist deciduous forest, three weeks
The explants were washed with soap in running tap water for 1 hour
Summary
20 explants and culture were maintained in each treatment and data (SE) “wMeicrerorperocpoardgeatdiounpotfoSf.oduulrciws”eeks of culture. The maximum number of shoot induction from the nodes of the explants was exhibited in a combination of BAP and IAA. Other species like S. transferred to half strength MS basal medium montevidiensis shows adequate response towards shoot supplemented with different concentrations of neither IBA regeneration in MS medium in the presence of BAP nor IAA (0.0, 0.25, 0.50 and 1.0 mg/l) and 3% (w/v) (Escandon et al, 2005). The rooted plants were removed length resulted when KN and IBA were used at a from the culture tubes, washed with sterile distilled water, concentration of 2.0 and 0.5 mg/l respectively (Fig. 1)
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