Abstract
A flow injection (FI) spectrophotometric method for the determination of olive oil anisidine value (AV) is presented. A 40-l aliquot of 10% (w/v) olive oil samples in 2-propanol are injected in a p-anisidine in 2-propanol/acetic acid stream. The reagents are premixed in the FI system using a two-line manifold. Detection is achieved by monitoring absorbance continuously at 350 nm. The proposed FI method achieves a high analysis rate (110 samples/h), minimizes solvent consumption (2 ml 2-propanol and 0.4 ml acetic acid per analysis) and compares well with the manual spectrophotometric method (relative difference 0.5–6.8% for the analysis of 25 olive oil samples). Precision was found to be better than 1% relative standard deviation (R.S.D.; n=7). The linear range was 6–85 AV and is suitable for analysis of abused olive oils.
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