Direct multiplex allele-specific PCR amplification for ABO genotyping from whole blood, hair root and buccal cell

Abstract

BackgroundABO genotyping is an increasingly being used for accurate prediction of blood transfusion, organ transplantation and forensic application. Traditional methods require DNA extract and purification which is time-consuming labor-intensive and limit the trace samples detection. MethodsPresent study developed the multiplex allele-specific polymerase chain reaction (AS-PCR) method to identify the six major genotypes of ABO blood groups in two tubes reaction. The samples of whole blood, buccal cell and hair root from 30 volunteers were examined. ResultsAll genotyping results were interpreted and agreed with the serologic findings. The modified AS-PCR method was able to determine ABO genotyping using direct blood and non-invasive samples including buccal cell and hair root. ConclusionsThe modified technique has a high specific and sensitive, rapid and cost-efficiency of genotyping method which might be valuable for transfusion medicine in a clinical laboratory and personal identification in forensic investigation.