Abstract
SynopsisWe present the first experimental demonstration of Z↔E photoisomerization the GFP chromophore anion, HBDI−, in the gas phase. In the single photon absorption regime, the photoisomerization action spectra show two maxima at 480 nm and 455 nm. In the multiphoton absorption regime, photodissociation and photodetachment channels modify the appearance of the photoisomerization band. This work provides a new approach to characterize photoisomerization pathways in biomolecular ions.
Highlights
Direct monitoring of photon induced isomerization, dissociation and electron detachment of the green fluorescent protein chromophore anion
Synopsis We present the first experimental demonstration of Z↔E photoisomerization the green fluorescent protein (GFP) chromophore anion, HBDI−, in the gas phase
While many insights into the nature and dynamics of photoinduced fragmentation and electron emission have been gained, the possibility of Z↔E photoisomerization in HBDI−, a deactivation pathway competing with photodissociation and photodetachment, has received less attention, mainly due to the challenges associated with its experimental detection
Summary
Direct monitoring of photon induced isomerization, dissociation and electron detachment of the green fluorescent protein chromophore anion. To cite this article: Eduardo Carrascosa et al 2017 J. Synopsis We present the first experimental demonstration of Z↔E photoisomerization the GFP chromophore anion, HBDI−, in the gas phase.
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