Direct infiltration effects of dimethyl sulfoxide into ovarian bursas on spontaneous ovulation and the estrous cycle of the rat: antagonism on the type 2 dopaminergic receptor

Abstract

We studied effects of microinjection (MI) into ovarian burses (OB) of infiltering agent dimethyl sulfoxide (DMSO) on number of ova shed (NOS) and estrous cycle (EC) of adult female rats with fourth day regular estrous cycles (REC), and we compared these effects with a MI of 100µg sulpiride solution in DMSO. Between 13:00- 14:00h of different days of EC, CER groups received 20µL of pure DMSO into both OB; the sham groups received MI with 20µL distillated water (H2O) into each OB. All rats with MI performed and an intact cyclic rat group were autopsied in the morning of next estrous vaginal shown (EVS). Both DMSO and H2O MI does not modify the EC duration and NOS. Other groups of cyclic rats received a MI with 20µL sulpiride solution into each OB and were autopsied at next EVS. Just the sulpiride MI performed on diestrous-1 day delayed 24h the next EVS, but that don’t occur in other sulpiride groups. The NOS were not modified in all experimental group. The DMSO direct administration in ovarian tissue does not affect gonadal primordial functions and its use is recommended like an infiltrating agent of non-polar drugs.