Abstract
A rapid and direct radioimmunoassay of urine estrone conjugate (E 1C) was developed. Urine samples were taken from Large White sows by inserting sponges in the vagina which were expelled during micturition. Analysis of samples collected daily from 13 sows between 2 and 40 days after service showed that high urine E 1C levels occurred between day 20 and 30 after insemination in pregnant sows; maximum concentrations were observed on day 25 (79.4 ± 11.7 ng/ml, X ± s.e.m. ). In this period E 1C levels never exceeded 1 ng/ml in nonpregnant sows. A single urine sample was then taken from 84 sows, 25 days after insemination, to check the accuracy of E 1C estimation as a test for early pregnancy diagnosis. Seventy-six of the examined animals were correctly diagnosed, giving an overall accuracy of 90.4%. No relationship was found between litter size and urine E 1C levels.
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