Abstract
The direct electrochemistry of hemoglobin (Hb) molecules adsorbed on bare indium tin oxide (ITO) electrodes was investigated by voltammetric methods. A pair of redox peak currents, which is attributed to a heme Fe(III)/Fe(II) couple in Hb molecules, was found at Hb-adsorbed ITO electrodes in phosphate-buffered saline (PBS; pH 7.4) solutions. Slab optical waveguide (SOWG) spectroscopy, which is one of the attenuated total reflection (ATR) techniques, was employed to observe the Soret band of Hb molecules adsorbed on the bare ITO electrode surfaces. The obtained spectra indicated that directly adsorbed Hb molecules were not significantly denatured on the bare ITO electrode surfaces.
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