In Situ Observation of Time Dependent Electrochemical Activity of Cytochrome c at Bare Indium-Tin-Oxide Electrodes by Cyclic Voltammetry and Slab Optical Waveguide Spectroscopy

Abstract

In situ observation of electrochemical activity and time dependent characteristics of cytochrome c (cyt c) was carried out in 0.01 M phosphate buffered saline (PBS, pH 7.4) containing 20µM cyt c solutions at bare indium-tin-oxide (ITO) electrodes by using a cyclic voltammetry (CV) and a slab optical waveguide (SOWG) spectroscopy. The bare ITO electrodes could retain the electrochemical activity of cyt c in the PBS solutions, indicating the great advantage of using ITO electrodes against other electrode materials, such as gold (Au). The CV curves and simultaneously observed the time-resolved SOWG absorption spectra in the consecutive cycles implied that the cyt c molecules could retain its own electrochemical function for a long time.