Abstract
Direct and Simple Method for Mesenchymal Stem Cells Isolation, Culturing and Detection
Highlights
Mesenchymal Stem cells (MSCs) are differentiated mainly from mesoderm embryonic germ layer give rise to skeletal and connective tissue, and able to differentiate into neural and myogenic cells [1].It’s isolated from many adult tissues like stromal bone marrow, marrow aspirates, periosteum, trabecular bone, synovium, skeletal muscle and deciduous teeth and adipose tissue these cells have capacity to differentiate into connective tissue including bone, fat, cartilage and muscle
Bone marrow mesenchymal stem cells (BM-MSCs) are the best adult stem cells that can be used for autologous regenerative medicine
Positive selection only selected CD34+ cells were others sub cultured in a 25 cm2 culture flask [6], but different procedure used for their purification like negative isolation in which Adherent cells from adult C57Bl/6J mouse bone marrow were depleted of granulo-monocytic cells and subsequently allowed to grow on fibronectin-coated dishes in presence of fetal bovine serum and growth factors [7]
Summary
Mesenchymal Stem cells (MSCs) are differentiated mainly from mesoderm embryonic germ layer give rise to skeletal and connective tissue, and able to differentiate into neural and myogenic cells [1].It’s isolated from many adult tissues like stromal bone marrow, marrow aspirates, periosteum, trabecular bone, synovium, skeletal muscle and deciduous teeth and adipose tissue these cells have capacity to differentiate into connective tissue including bone, fat, cartilage and muscle. Positive selection only selected CD34+ cells were others sub cultured in a 25 cm2 culture flask [6], but different procedure used for their purification like negative isolation in which Adherent cells from adult C57Bl/6J mouse bone marrow were depleted of granulo-monocytic cells and subsequently allowed to grow on fibronectin-coated dishes in presence of fetal bovine serum and growth factors [7].
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