Abstract
Direct analysis in real time (DART) is a recently developed ambient ionization technique for mass spectrometry to enable rapid and sensitive analyses with little or no sample preparation. After swab-based field sampling, the organothiophosphate malathion was analyzed using DART-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Mass resolution was documented to be over 800,000 in full-scan MS mode and over 1,000,000 for an MS/MS product ion produced by collision-induced dissociation of the protonated analyte. Mass measurement accuracy below 1 ppm was obtained for all DART-generated ions that belonged to the test compound in the mass spectra acquired using only external mass calibration. This high mass measurement accuracy, achievable at present only through FTMS, was required for unequivocal identification of the corresponding molecular formulae.
Highlights
Direct analysis in real time (DART) is an ambient ionization technique for mass spectrometry developed a decade ago to enable rapid and sensitive analyses with little or no sample preparation [1]
Application of tandem mass spectrometry (MS/MS) at ultrahigh resolution made possible by Fourier transform mass spectrometry (FTMS) has not been exploited using DART
With focus on homeland security application, we report here the evaluation of Fourier Itnrta. nJ.sMfoolr.mSci.io20n16c, y17c,l1o1t6ron resonance (FT-ICR) mass spectrometry to detect sulfur-containing s2mofa8ll molecules at high resolution to achieve adequate measurement accuracy (MMA) using both DART–MS and DART–MS/MS techniques
Summary
Direct analysis in real time (DART) is an ambient ionization technique for mass spectrometry developed a decade ago to enable rapid and sensitive analyses with little or no sample preparation [1]. The technique is attractive for homeland security purposes, where accurate detection of chemical warfare agents is critical to make informed decisions [3]. High resolution and accurate mass measurement are generally required for application of the technique to real-life samples [4]. Application of tandem mass spectrometry (MS/MS) at ultrahigh resolution made possible by Fourier transform mass spectrometry (FTMS) has not been exploited using DART. With focus on homeland security application, we report here the evaluation of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry to detect sulfur-containing small molecules at. NJ.sMfoolr.mSci.io20n16c, y17c,l1o1t6ron resonance (FT-ICR) mass spectrometry to detect sulfur-containing s2mofa8ll molecules at high resolution to achieve adequate MMA using both DART–MS and DART–MS/MS techniques. M/∆M exceeded 1,000,000 for this fragment ion, when the desired resolution at m/z 400 was set to 500,000, as displayed in the inset of Figure 3
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