Abstract
The diosgenin concentrations in a Dioscorea polygonoides tuber collection from the Eje Cafetero (EC, Colombia) were determined by HPLC and their percentages ranged from 0.02 to 2.64%. The highest diosgenin concentration (2.64%) was obtained in a tuber collected near Salento (Quindio). The average of diosgenin recovery was 97%. Diosgenin was identified by gas chromatography-mass spectrometry (GC-MS) and by coelution with authentic diosgenin standard in both HPLC and GC-MS techniques. The results of this study are important since comparatively they are higher than most of those reported on other medicinal Dioscorea species, making Dioscorea polygonoides a potential new source of diosgenin.
Highlights
The most important families that biosynthesize steroidal saponins are Agavaceae, Dioscoreaceae and Liliaceae.[1]
Several Dioscorea species are economically important as staple food,[3,4] and others are used for the production of steroidal saponins, which on hydrolysis render sapogenins, such as diosgenin (Figure 1), which is important as starting material for the production of corticosteroids, sexual hormones, oral contraceptives as well as other steroids via hemisynthesis.[5,6,7]
The aim of this study was to quantify by high pressure liquid chromatography (HPLC) the levels of diosgenin in a collection of seventy four tubers of D. polygonoides from different localities of the Eje Cafetero (EC, Colombia)
Summary
The most important families that biosynthesize steroidal saponins are Agavaceae (genus Agave), Dioscoreaceae (genus Dioscorea) and Liliaceae (genera Allium, Asparagus, Lilium).[1]. Diosgenin Quantification by HPLC in a Dioscorea polygonoides Tuber Collection
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