Dihydropyridines as potential α-amylase and α-glucosidase inhibitors: Synthesis, in vitro and in silico studies.

Abstract

Dihydropyridine derivatives 1-31 were synthesized via one-pot solvent free condition and screened for in vitro against α-amylase and α-glucosidase enzyme. The synthetic derivatives 1-31 showed good α-amylase inhibition in the range of IC50=2.21±0.06-9.97±0.08µM, as compared to the standard drug acarbose (IC50=2.01±0.1µM) and α-glucosidase inhibition in the range of IC50=2.31±0.09-9.9±0.1µM as compared to standard acarbose (IC50=2.07±0.1µM), respectively. To determine the mode of binding interactions of synthetic molecules with active sites of enzyme, molecular docking studies were also performed. Different spectroscopic techniques such as 1H, 13C NMR, EI-MS, and HREI-MS were used to characterize all the synthetic compounds.