Abstract
Detection of diarrhoeagenic Escherichia coli in stool specimens cannot be performed easily in most routine laboratories. This study describes a rapid hybridization assay for identification of enterovirulent Escherichia coli in faecal samples. DNA fragment probes specific for enterotoxigenic Escherichia coli, Vero cytotoxin producing Escherichia coli and enteropathogenic Escherichia coli were labelled with digoxigenin and produced in large quantities by polymerase chain reaction. Stool samples were cultured overnight, replica-plated and hybridized with five different probes. This method gives satisfactory results, requires a minimum number of bacteria subcultures and may be used routinely in the laboratory.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
