Abstract
The scale and complexity of images collected in biological microscopy have grown enormously over the past 30 years. The development and commercialization of multiphoton microscopy has promoted a renaissance of intravital microscopy, providing a window into cell biology in vivo. New methods of optical sectioning and tissue clearing now enable biologists to characterize entire organs at subcellular resolution. New methods of multiplexed imaging support simultaneous localization of forty or more probes at a time. Exploiting these exciting new techniques has increasingly required biomedical researchers to master procedures of image analysis that were once the specialized province of imaging experts. A primary goal of the Indiana O’Brien Center has been to develop robust and accessible image analysis tools for biomedical researchers. Here we describe biomedical image analysis software developed by the Indiana O’Brien Center over the past 25 years.
Highlights
Over the past 200 years, biological microscopy has evolved from a largely descriptive technique, documented with pictures and verbal descriptions, into a legitimately quantitative research approach
In our first forays into intravital microscopy we immediately discovered that tissue motion, derived primarily from respiration, represented a significant challenge to high resolution in vivo imaging
1http://web.medicine.iupui.edu/ICBM/software of pervasive image distortion in time series and 3D intravital Core of the Indiana O’Brien Center developed novel software microscopy, the students from the laboratory of Edward Delp, to retrospectively correct intravital microcopy images that were a Purdue University investigator of the Digital Image Analysis compromised by motion artifacts
Summary
Over the past 200 years, biological microscopy has evolved from a largely descriptive technique, documented with pictures and verbal descriptions, into a legitimately quantitative research approach. 1http://web.medicine.iupui.edu/ICBM/software of pervasive image distortion in time series and 3D intravital Core of the Indiana O’Brien Center developed novel software microscopy, the students from the laboratory of Edward Delp, to retrospectively correct intravital microcopy images that were a Purdue University investigator of the Digital Image Analysis compromised by motion artifacts.
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