Abstract
A simple chromatographic tube test is described to differentiate polio-virus type 3 strains labelled with32P on aluminiumhydroxide. There are differences between Leon 12 a1b (and progeny after human passage) and epidemic strains 1963 (Baden-Wurttemberg, SW-Germany) facilitating identification of virus strains isolated after mass vaccination with Leon 12 a1b. The marker of strain Leon 12 a1b (50% elution at 0.05–0.06 mol. phosphate) examined in 43 strains after human passage was found to be stable even 3–4 weeks after vaccination. 24 type 3 epidemic strains isolated during 3 months, before vaccination had begun, behave differently (50% elution at 0.10–0.13 mol. phosphate). The standard deviation of single values from the median value is 10%. 18 wild strains isolated in the years 1958–1962 behave like wild strains 1963, 5 strains from 1961, however, elute at 0.21 mol. phosphate. A fourth distinct type of chromatographic behavior was found in a group of type 3 strains consisting of Leon wild strain, WFX and Lederle Fox with 50% elution at 0.02–0.03 mol. phosphate. Strains belonging to this latter group behave chromatographically inhomogeneous. Chromatography on aluminiumhydroxide with32P-labelled virus seems to be a very convenient method to characterize poliovirus by differences in surface structures.
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