Abstract
This study was undertaken to examine whether human early round spermatids will differentiate in an in vitro coculture with Vero cells. A total of 1450 and 400 isolated early round spermatids mechanically collected from two non-obstructive and three obstructive azoospermic men with a normal karyotype were cocultured on Vero cell monolayers in minimum essential medium plus 10% fetal bovine serum, with or without 50 or 100 IU/L FSH and 1 or 10 μmol/L testosterone, at 32.5°C, in an environment of 5% CO2 in air. Morphological changes of the spermatids were observed microscopically. After 7 days of coculture, almost half (40-50%) of the round spermatids from both non-obstructive and obstructive azoospermic men resumed spermiogenesis in vitro. Only cells from the latter patients gave rise to spermatozoa, a few of which had a motile flagellum. Low concentrations of FSH and testosterone increased the percentage of in vitro spermiogenesis. Isolated round spermatids can resume spermiogenesis in vitro when cocultured on a Vero cell monolayer.
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