DIFFERENTIATION OF HUMAN OLFACTORY BULB NEURAL STEM CELLS INTO OLIGODENDROCYTES

Abstract

Human olfactory bulb neural stem cells (OBNSCs) are multipotent cells that are capable of differentiation into neurons, astrocytes, and oligodendrocytes. In the present study, we investigated the ability of human OBNSCS to differentiate into oligodendrocytes using RT-PCR and immunocytochemistry. In the presence of fetal calf serum, all-trans retinoic acid (RA), triiodothyronine (T3), and cyclic adenosine monophosphate (cAMP), OBNSCS were differentiated into oligodendrocytes precursors as evidenced by induction and repression of oligodendrocytes chondroitin sulphate proteoglycan (NG2) and nestin genes, respectively. These finding were confirmed at the protein level using fluorescent immunocytochemistry (FICC) according to standard protocol. Taken together, the present study highlighted the ability of OBNSC to differentiate into oligodendrocytes, a finding that indicates their possible use as a promising candidate for cell-based therapy for neurodegenerative and traumatic diseases associated with myelin impairments. Abbreviations: Human olfactory bulb neural stem cells (OBNSCs), retinoic acid (RA), triiodothyronine (T3), cyclic adenosine monophosphate (cAMP), pro-oligodendrocytes chondroitin sulphate proteoglycan (NG2), immunocytochemistry (FICC).