Abstract
Objective To find an optimal way to induce differentiation of adipose-derived stem cells (ASCs) into epidermal cells.Methods In co-culture group,HaCaT cells and ASCs were co-cultured by using Transwell apparatus.In epidermal growth factor (EGF) induction group,EGF was added into the culture medium of ASCs.After culture for 3 and 6 days,ASCs were detected by counting the number and dying the markers of epidermal cells such as cytokeratin 19 (CK-19),integrin β1 and pan-cytokeratin (Pan-CK).Results The positive rate of CK-19,integrin β1 and Pan-CK in ASCs was (25.8 ±4.1 )％,( 29.2 ± 3.9) ％ and ( 18.3 ± 2.7 ) ％ respectively at 3rd day after culture by co-culture method,which was significantly higher than in EGF induction group (P ＜ 0.05).The positive rate of CK-19,integrin β1 and Pan-CK in ASCs was ( 34.1 ± 5.7) ％,(42.8 ± 4.3 ) ％ and (29.4 ± 3.3 ) ％ respectively at 6th day after culturc by co culturc method,which was significantly higher than in EGF induction group ( P ＜0.01 ).The number of epidermal cells obtained by co-culture method was significantly more than that in EGF induction group.Conclusion Co-culture of ASCs with HaCaT cells is an optimal method to promote the differentiation of ASCs into epidermal cells. Key words: Adipose-derived stem cells; Epidermal cells; Induction; Differentiation
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