Differential serotonin responses in the skeletal muscle microcirculation

Abstract

Closed circuit television microscopy was used to quantitate in vivo responses of small vessels in the rat cremaster muscle to topically applied serotonin. Sprague-Dawley rats were anesthetized with a combination of urethane (800 mg/kg) and alpha-chloralose (60 mg/kg). The cremaster muscle with intact circulation and innervation was suspended in a bath which had controlled pH, pCO 2, and pO 2. Microvascular diameter of first order arterioles and venules and fourth-order arterioles were measured from the television monitor while serotonin (10 −9M-10 −4M) was added to the bath. Fourth-order arterioles (3–11 μm diameter) dilated to a maximum of 267% of their control value with a serotonin concentration of 10 −6M. Serotonin (10 −4M) constricted first-order arterioles (78–121 μm) to 61% of their control value. The threshold concentration (10 −8M) for a serotonin-induced dilation of fourth-order arterioles was 1000 fold less than the threshold concentration (10 −5M) for serotonin-induced constriction of first-order arterioles. Serotonin (10 −8M-10 −4M) did not alter the diameter of first-order venules (115–195 μm) from the control value. The dose-dependent constriction of first-order arterioles and dose-dependent dilation of fourth-order arterioles by serotonin appear to be independent of each other. In addition, the lack of constriction of first-order venules suggests a heterogenous distribution of serotonin receptors and that the predominate control mechanisms are different at different levels of the arteriolar and venous microcirculation of rat skeletal muscle.