Differential Role of 6-Benzylaminopurine in Potato Tissue Culture

Abstract

Phytohormones are the derivatives of secondary metabolic pathways. They are of diverse physiological role, molecular action and metabolism but they do cross talk during hormonal signaling pathways. During in vitro micropropagation and regeneration studies in potato (Solanum tuberosum L.), so called berries like structures were observed. In these studies the effect of gibberellic acid (GA3), NAA (auxin) and BAP (cytokinins) were being investigating on in vitro micropropagation and in vitro regeneration of Solanum tuberosum L. (potato). In in vitro micropropagation study when potato tubers of cv. PRI-Red were treated with 0.3% GA3to induce sprouting and these sprouts were when cultured on micropropagation media containing 1.75, 2.75 mg/l BAP, then so called berries like structures were formed. Similarly, in in vitro regeneration study, when calli of potato cv. kuroda, induced on callus induction medium containing 4.5 mg/l 2-4D were when shifted on regeneration medium containing 4.75 mg/l BAP then same so-called berries like structures were formed. Hence histoanatomy of these berries like structure and microtubers were done which revealed that these are anatomically entirely different from microtubers.