Differential responses of the properties of soil humic acid and fulvic acid to nitrogen addition in the North China Plain

Abstract

Humus (HS) is an important component of soil organic matter. Humic acid (HA) and fulvic acid (FA) are two of the most important components of HS, as they substantially affect biogeochemical processes and the migration and transformation of pollutants in soil. Long-term nitrogen (N) addition can lead to changes in soil physical and chemical properties, affect the structural characteristics of soil HS (HA and FA), cause changes in the adsorption and migration of pollutants, and ultimately result in the continuous deterioration of the soil ecological environment. However, few studies have examined the effects of N addition on the structural characteristics of soil HS, including the responses of soil HA and FA to N addition. Here, we conducted a long-term positioning experiment with different levels of N addition (CK: 0 kg N ha−1 yr−1, LN: 100 kg N ha−1 yr−1, and HN: 300 kg N ha−1 yr−1) in typical farmland soils of the North China Plain to study the response of soil HA and FA to N addition. N addition altered the physical and chemical properties of soil (e.g., pH, SOC, TN, and enzyme activity), which affected the responses of the chemical structure, quality indexes, and composition distribution of soil HA and FA to N addition. Differences in the response to N addition between HA and FA were observed. The structural characteristics of FA were stronger in response to HN compared with those of soil HA. As the level of N added increased, soil FA degradation increased, the composition distribution changed, the aromatization degree and molecular weight decreased, and the molecular structure became simpler. The properties of soil HA did not significantly respond to N addition. Given increases in the global N input (N addition and N deposition), our results have implications for agricultural fertilization, soil management, and other activities.