Abstract
It is well known that Selenium plays important roles in human health. In this study, Selenium-enriched Pleurotus ostreatus (P.ostreatus) was cultivated by adding selenoarginine to substrate. Selenium content in mushrooms was measured by Atomic Fluorescence Spectrophotometer (AFS). We demonstrated that that selenium concentration in samples increased significantly and it had a significant positive relationship with selenium concentration in the cultivation material. Finally, we identified the total proteins extracted from ordinary and Se-enriched P.ostreatus by SDS-PAGE and 2-DE. We found there had no significant changes in protein content. Two-dimensional electrophoresis combining with mass spectrometry technology was applied to identify proteins that are differentially expressed in mushroom grown in the substrates with 44.26 mg/kg of Se. From 2D maps, 51 differentially expressed proteins were detected, 17 of those were up-expressed and 34 of those were down-expressed. 8 up-expressed proteins were analyzed by MALDI-TOF-MS/MS: 3 proteins were identified, one was hypothetical protein (gi|270056453) and the other two proteins are respectively NAD-dependent formate dehydrogenase (gi|164564766) and Aldo keto reductase (gi|628847367). These two proteins are mainly involved in substance and energy metabolism in the cell.
Highlights
P.ostreatus is one of the most appreciated mushrooms due to its delicious taste and high quantities of proteins, carbohydrates, minerals and vitamins (Baysal et al, 2003; Shah et al, 2004)
Se in P. ostreatus: P.ostreatus were able to absorb and accumulate Se when Selenoarginine was added in mushrooms cultivation
Selenoarginine can contribute to the development of stipe, at the same time inhibit the grows of pileus
Summary
P.ostreatus is one of the most appreciated mushrooms due to its delicious taste and high quantities of proteins, carbohydrates, minerals (calcium, phosphorus, iron) and vitamins (thiamin, riboflavin and niacin) (Baysal et al, 2003; Shah et al, 2004). P.ostreatus with nutritional and medicinal properties has significant value as an edible fungus which is commercially used world-wide. Since research has tended to focus on the dietary value of P.ostreatus, there is relatively little information pertaining to their antioxidant properties and their possible use to treat oxidative stress. Selenium has several physiological functions for enhancing immune system function, anti-aging and reducing cancer risk while transforming to organic selenium, especially combining with protein or polysaccharide (Finley, 2006). It has been a research hotspot in the fields of foodstuff science and medicine pharmacy to develop the edible and medicinal Se-enriched products in recent years
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