Abstract
This letter presents a practical application of a differential microstrip microwave sensor for label-free and realtime monitoring of Escherichia coli (E. coli) growth under different nutritional conditions on solid agar media. Two planar microstrip ring resonators coupled with a one-port power divider form the core of the sensing system. The sensing resonator operates at 1.509 GHz with an amplitude of -10.21 dB and the reference resonator operates at 1.798 GHz with an amplitude of -11.75 dB. The growth profile of E. coli at each glucose concentration in Luria-Bertani (LB) agar was derived by measuring the resonant amplitude variations (AA) and is further supported by microscopic images. A complete inhibition of growth was observed at 10% glucose concentration. This research expands the application of microwave sensors to investigate the effect of nutritional parameters on bacterial growth.
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