Abstract
Live-attenuated influenza virus (LAIV) vaccines provide broader cross-protection than whole-inactivated virus (WIV) vaccines against influenza A virus (IAV) in swine. However, standard correlates of protection, such as serum hemagglutination inhibition (HI) antibody titers, do not accurately predict cross-protective efficacy provided by LAIV vaccines. While the contribution of T cells to IAV immunity is appreciated, data comparing methods to evaluate IFN-γ production by IAV-specific T cells is limited. To understand the differential immunogenicity between LAIV and WIV vaccines as it relates to induction of T cell responses, IFN-γ production by peripheral T cells following antigen restimulation was assessed postvaccination. ELISpot assays used to enumerate IAV-specific IFN-γ secreting cells (SC) in peripheral blood 42 days post-vaccination (dpv) indicated that WIV-vaccinated pigs had a greater number of IFN-γ SC compared to LAIV vaccinated pigs, regardless of recall IAV used.
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