Abstract
SummaryStarvation for isoleucine inhibits chromosome, minichromosome and pBR322 DNA replication in a stringent strain ofE. coli, but does not do so in a relaxed mutant. Starvation for other amino acids inhibits either chromosome and minichromosome replication in both strains. From these results we conclude thatoriCand pBR322 replication are stringently regulated and that isoleucine seems not to be essential for the protein synthesis required at the initiation oforiCreplication. Deprivation of isoleucine in a Rel−strain gives rise to amplification of minichromosome and pBR322 with a better yield of the latter plasmid than that following treatment with chloramphenicol.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.