Abstract
Late leaf spot (LLS) is a major foliar disease in peanut (A. hypogaea L.) worldwide, causing significant losses of potential yield in the absence of fungicide applications. Mutants are important materials to study the function of disease-related genes. In this study, the mutant line M14 was derived from cultivar Yuanza 9102 treated with EMS. Yuanza 9102 was selected from an interspecific cross of cultivar Baisha 1016 with A. diogoi, and is resistant to several fungal diseases. By contrast, the M14 was highly susceptible to late leaf spot. RNA-Seq analysis in the leaf tissues of the M14 and its wild type Yuanza 9102 under pathogen challenge showed 2219 differentially expressed genes including1317 up-regulated genes and 902 down-regulated genes. Of these genes, 1541, 1988, 1344, 643 and 533 unigenes were obtained and annotated by public protein databases of SwissPort, TrEMBL, gene ontology (GO), KEGG and clusters of orthologous groups (COG), respectively. Differentially expressed genes (DEGs) showed that expression of inducible pathogenesis-related (PR) proteins was significantly up-regulated; in the meantime DEGs related to photosynthesis were down-regulated in the susceptible M14 in comparison to the resistant WT. Moreover, the up-regulated WRKY transcription factors and down-regulated plant hormones related to plant growth were detected in the M14. The results suggest that down-regulated chloroplast genes, up-regulated WRKY transcription factors, and depressed plant hormones related to plant growth in the M14 might coordinately render the susceptibility though there was a significant high level of PRs. Those negative effectors might be triggered in the susceptible plant by fungal infection and resulted in reduction of photosynthesis and phytohormones and led to symptom formation.
Highlights
The cultivated peanut (Arachis hypogaea L.) has been known as one of the most important oil seed crops worldwide, providing high quality edible oil and high protein content in the seed
We have statistically tested three phenotypic traits related with plant growth, i.e. main stem height, 100 pod weight, and 100 seed weight
All raw data were deposited in the National Center for Biotechnology Information (NCBI) as accession SRX3088919 and SRX3088920
Summary
The cultivated peanut (Arachis hypogaea L.) has been known as one of the most important oil seed crops worldwide, providing high quality edible oil and high protein content in the seed. Late leaf spot (LLS) caused by Phaeoisariopsis personata Multiple applications of fungicides can effectively control of leaf spot disease, fungicides spray could cause environmental pollution and increase production costs [5]. The most economical and environment-friendly management strategy to control LLS disease is to develop and utilize resistant varieties in the crop production [6,7,8]. Several diploid wild species and tetraploid cultivars have showed high levels of resistance to LLS [11,12]. Utilization of these resistance sources has developed superior varieties resistant to LLS, but conventional resistance breeding is time-consuming [13]. To better understanding of molecular mechanism governing LLS resistance, genome-wide transcriptom profiles are necessary
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