Differential expression pattern of Annexin A6 in chick neural crest and placode cells during cranial gangliogenesis

Abstract

The cranial trigeminal and epibranchial ganglia are components of the peripheral nervous system that possess an important somatosensory role. These ganglia arise from the intermixing and coalescence of two different migratory cell types, neural crest cells and neurogenic placodes cells, and thus typify the phenomena of cell migration and intercellular interactions for their creation. The underlying molecular mechanisms of ganglia formation, however, are still poorly understood. To address this, we have analyzed the spatio-temporal expression profile of Annexin A6 during chick gangliogenesis, as Annexin proteins play important, conserved roles in ganglia development and physiology. We observe Annexin A6 protein in cranial neural crest cells prior to, during and after their emergence from the neural tube. Fully migratory cranial neural crest cells, however, are devoid of Annexin A6. Interestingly, we note Annexin A6 protein in trigeminal and epibranchial placode cells as these cells ingress from the ectoderm to initiate ganglia formation. This expression is also maintained in the sensory placodes later on when they coalesce with neural crest cells to assemble the cranial ganglia. These results suggest that the dynamic expression of Annexin A6 in various embryonic cell types may allow Annexin A6 to serve distinct functions throughout embryonic development.