Abstract
To clarify the molecular basis for the prostaglandin (PG) mediated effects in adipose cells at various stages of their development, expression of mRNAs encoding receptors specific for prostaglandin E 2, F 2α and I 2 (i.e. EP, FP, and IP receptors) was investigated in differentiating clonal Ob1771 pre-adipocytes, as well as in mouse primary adipose precursor cells and mature adipocytes. We have further characterized the differential expression of mRNAs encoding three subtypes of the EP receptor, i.e. EP 1, EP 3, and EP 4, and examined the expression of mRNAs encoding the three isoforms (α, β, and γ) of the EP 3 receptor. Altogether the results show that the expression of IP, FP, EP 1, and EP 4 receptor mRNAs was considerably more pronounced in pre-adipose cells than in adipose cells, mRNAs encoding the α, β, and γ isoforms of the EP 3 receptor were all exclusively expressed in freshly isolated mature adipocytes. These data may indicate that PGI 2, PGF 2α, and PGE 2 may interact directly with specific receptors in pre-adipose cells, whose transduction mechanisms are known to affect maturation related changes. In mature adipocytes, however, the equipment of mRNAs encoding the EP 3 receptor isoforms is in agreement with the well known effect of PGE 2 on adenylate cyclase and lipolysis in mature adipocytes.
Published Version
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