Differential Expression of mRNAs in Peripheral Blood Related to Prodrome and Progression of Alzheimer's Disease.

Weishuang Xue,Jinwei Li,Kailei Fu,Weiyu Teng,Antoni Camins

doi:10.1155/2020/4505720

Weishuang Xue, Jinwei Li + Show 3 more

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https://doi.org/10.1155/2020/4505720

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Abstract

Alzheimer's disease (AD) is a chronic progressive neurodegenerative disease that affects the quality of life of elderly individuals, while the pathogenesis of AD is still unclear. Based on the bioinformatics analysis of differentially expressed genes (DEGs) in peripheral blood samples, we investigated genes related to mild cognitive impairment (MCI), AD, and late-stage AD that might be used for predicting the conversions. Methods. We obtained the DEGs in MCI, AD, and advanced AD patients from the Gene Expression Omnibus (GEO) database. A Venn diagram was used to identify the intersecting genes. Gene Ontology (GO) and Kyoto Gene and Genomic Encyclopedia (KEGG) were used to analyze the functions and pathways of the intersecting genes. Protein-protein interaction (PPI) networks were constructed to visualize the network of the proteins coded by the related genes. Hub genes were selected based on the PPI network. Results. Bioinformatics analysis indicated that there were 61 DEGs in both the MCI and AD groups and 27 the same DEGs among the three groups. Using GO and KEGG analyses, we found that these genes were related to the function of mitochondria and ribosome. Hub genes were determined by bioinformatics software based on the PPI network. Conclusions. Mitochondrial and ribosomal dysfunction in peripheral blood may be early signs in AD patients and related to the disease progression. The identified hub genes may provide the possibility for predicting AD progression or be the possible targets for treatments.

Highlights

Alzheimer’s disease (AD) is a chronic neurodegenerative disease with an insidious onset and progressive development
KEGG pathway analysis revealed that the differentially expressed genes (DEGs) were mainly enriched in ribosome, oxidative phosphorylation, Parkinson disease, nonalcoholic fatty liver disease (NAFLD), and Alzheimer’s disease in the Mild cognitive impairment (MCI) group and AD group (Figures 1(b) and 1(c), Supplementary Tables 2 and 3) and in herpes simplex virus 1 infection, p53 signaling pathway, ubiquitinmediated proteolysis, cell cycle, and prostate cancer in the advanced AD group (Figure 1(d))
After comparing these three groups of DEGs, we obtained the DEGs related to MCI, AD, and advanced AD

Summary

Introduction

Alzheimer’s disease (AD) is a chronic neurodegenerative disease with an insidious onset and progressive development. It is characterized by comprehensive dementia manifestations such as memory impairment, aphasia, apraxia, and loss of recognition. AD, affecting a large number of people and families, places a heavy burden on society and the economy [1]. With continued improvements in healthcare and the increase in the human lifespan, the proportion of older people in the population continues to increase [2]. Some MCI patients tend to be stable or even revert to a “healthy” status [3]. Poor life quality was observed in patients with late-stage AD, and the mortality rate is about 25% within 6 months [5].

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