Abstract
Objective To observe the changes of Sprague- Dawley rat sex hormone levels influence the expression of the methionine adenosyltransferase- 1A(MAT1A) in rats liver tissues. Methods To select 36 sexually mature Sprague- Dawley rats, build a SD rat model of sex hormone concentration gradient difference(male and female have three groups, each group of 6). Using enzyme linked immunosorbent assay(ELISA) method to measure the sex hormone concentrations of each group rats and the expressions of MAT1A were examined by the Western blotting and realtime quantitative polymerase chain reaction(RT-qPCR). Results ELISA's results: Estrogen concentrations of FM0 group is(43.125±11.066) ng/L, FM+ group is 0 ng/L, FM+ T group is (31.738±7.142) ng/L(P FM+> FM+ T, with a mean of 0.471 4> 0.133 4> 0.066 0(P< 0.01), males MAT1A expression is M0< M+< M+ E, with an average of 0.029 8< 0.068 7< 0.205 5(P< 0.01).Western blotting: Male and female liver MAT1A protein- expression in rats showed consistent result with the outcome of RT-qPCR. Conclusion The expression of MAT1A protein in rats have obvious gender differences, the expression of high levels of estrogen is advantageous to the MAT1A, whereas androgen inhibits the expression of MAT1A, while sex hormones through regulating MAT1A sex differences in expression to play an important role in gender differences in distribution of liver cancer. Key words: Methionine adenosyltransferase- 1A; Estradiol; Gender differences; Liver
Published Version
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