Abstract
BackgroundPolycystic ovary syndrome (PCOS) is a common endocrine disease in women of reproductive age. Multiple studies have shown that long non-coding RNAs (lncRNA) and microRNAs (miRNA) play a role in PCOS. This study aimed to explore the role and molecular mechanism of lncRNA -Regulator of reprogramming (lncROR) in PCOS.ResultsExpression level of lncROR in PCOS patients was up-regulated, while level of miR-206 was down-regulated in comparison with control group (P < 0.001). Logistics regression analysis showed that lncROR and miR-206 were independent predictors of PCOS. The ROC curve showed that lncROR had a high diagnostic value for PCOS with an AUC value of 0.893. Pearson correlation coefficient indicated that the expression level of miR-206 was negatively correlated with the level of lncROR. CCK-8 assay and apoptosis assay revealed that downregulation of lncROR up-regulated the expression of miR-206, thereby inhibiting cell proliferation and promoting cell apoptosis. However, silencing the expression of miR-206 reversed the above effects caused by down-regulation of lncROR expression. Luciferase reporter gene assay suggested that there was a target relationship between lncROR and miR-206. VEGF was proved to be the target gene of miR-206.ConclusionsHighly expressed lncROR indirectly up-regulated the expression of VEGF by down-regulating the expression of miR-206, thereby promoting the proliferation of KGN cells and inhibiting apoptosis, and further promoting the development of PCOS.
Highlights
Polycystic ovary syndrome (PCOS) is the most common endocrine disease in women of childbearing age [1], which is a group of clinical syndromes characterized by reproductive disorders, endocrine abnormalities and metabolic disorders [2]
There was no significant difference in age, follicle-stimulating hormone (FSH), estradiol (E2) and prolactin (PRL) levels between the PCOS group and the control group
The BMI of the PCOS group was increased compared with the control group (P < 0.05), while the level of luteinizing hormone (LH) and testosterone (TES) were significantly increased in comparison with control group (P < 0.001)
Summary
Polycystic ovary syndrome (PCOS) is the most common endocrine disease in women of childbearing age [1], which is a group of clinical syndromes characterized by reproductive disorders, endocrine abnormalities and metabolic disorders [2]. The pathogenesis of PCOS is still unclear, but some studies have been proved that genetic [5] and environmental factors [6] play a role in the occurrence and development of the disease. Polycystic ovary syndrome (PCOS) is a common endocrine disease in women of reproductive age. Multiple studies have shown that long non-coding RNAs (lncRNA) and microRNAs (miRNA) play a role in PCOS. This study aimed to explore the role and molecular mechanism of lncRNA -Regulator of reprogramming (lncROR) in PCOS
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