Differential expression of circulating miR-21, miR-142-3p and miR-155 in renal transplant recipients with impaired graft function.

Abstract

The discovery of circulating microRNAs (miRNAs), as potential noninvasive diagnostic biomarkers, has opened new avenues of research for identifying patients with chronic failure in renal transplantation. The present study aimed to investigate the expression levels of four immune-related miRNAs (miR-21, miR-31, miR-142-3p and miR-155) in plasma samples of renal recipients. The plasma expression levels of the miRNAs were evaluated by quantitative real-time PCR (qPCR) in 53 renal recipients with long-term stable allograft function, SGF (N=27), and with biopsy-proven interstitial fibrosis and tubular atrophy (IFTA) (N=26) and also healthy controls (N=15). The possible correlation between clinical parameters and the circulating miRNAs and the receiver-operating characteristic (ROC) analysis were performed. Our results showed that expression of miR-21 (p=0.023), miR-142-3p (p=0.048) and miR-155 (p=0.005) was significantly upregulated in plasma samples of recipients with IFTA in comparison with SGF and healthy control groups. Concentration of miR-21 (∆Ct value) in plasma was negatively correlated with creatinine (r=-0.432, p=0.028) and positively correlated with eGFR (r=0.423, p=0.031). The multivariate ROC curve analysis indicated that miR-21, miR-142-3p and miR-155 in plasma samples could discriminate almost most of the IFTA patients (area under curve=0.802, sensitivity=81%, specificity=92%). Our data suggested that altered expression of miR-21, miR-142-3p and miR-155 in plasma samples may be associated with renal dysfunction and can be used for graft monitoring.