Abstract
The plasma membrane mediates many essential biological functions including cell-cell interactions, molecular transport, and signal transduction. Many membrane associated proteins are overexpressed in disease (eg cancer), and the ectodomains proteolytically shed. Several clinically used biomarkers have membranous origin including, CEA in colorectal cancer and PSA in prostate cancer. For this reason membrane proteomes are of interest in the field of biomarker discovery. As part of the AOHUPO membrane protein initiative, we report a solubility-based phase partitioning of mouse liver microsomes, using the non-ionic detergent Triton X-114. The aqueous, detergent, and pellet-insoluble fractions were obtained and subjected to SDS-PAGE in combination with LCMS/MS.
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