Abstract
Identification of differentially expressed genes can be done by cDNA-AFLP analysis. Hard bunch phenomena are not widely studied. The aim of this research was to identify and characterize regions specific to the gene coding sequences involved in the hard bunch phenomena in oil palm fruits. RNA was isolated from three types of ripe oil palm fruits including normal, black and hard bunch fruits. cDNAs were amplified using 16 AFLP primer pairs combinations. The result of a BLAST comparison showed that some of the resulting transcripts have sequence similarity with the existing database sequences, such as a WRKY transcription factor, peroxidase, actin depolymerising factor and glutamine synthetase allegedly associated with hard bunch phenomenon induced by drought stress. However, some transcripts could not be classified since they did not exhibit significant similarity to any existing database sequences. This is might be the transcripts were less conserve region and or 5’ or 3’ UTR region.
Highlights
Hard bunch is the non-shed ripe oil palm fruits and some fruits remain attached to the bunch after the process of separating the fruit from the bunch at the thresher of the mill
Water deficit was triggered by low rainfall and supported by other climatic elements that cause the increase of the plant transpiration rate, such as the relative humidity, the wind speed and the distribution of sunshine hours (Roberdi, 2014)
The aim of this research was to identify and characterize a differential expressed genes involved in hard bunch phenomena in oil palm fruits
Summary
Hard bunch is the non-shed ripe oil palm fruits and some fruits remain attached to the bunch after the process of separating the fruit from the bunch at the thresher of the mill. Hard bunch phenomena causes two problems in oil palm industry. Hard bunch caused reducing oil extraction ratio in oil palm plantations and low Crude Palm Oil (CPO) quality do to high of Free Fatty Acid (FFA) content. This phenomenon occurred when the plants were exposed to water deficit severely during six to nine months before harvesting fruit bunches. Water deficit was triggered by low rainfall and supported by other climatic elements that cause the increase of the plant transpiration rate, such as the relative humidity, the wind speed and the distribution of sunshine hours (Roberdi, 2014). Water deficit combined with mechanical stress can reduced sex ratio in oil palm (Ajambang et al, 2015)
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