Abstract
While developing a radioimmunoassay for the measurement of 6-keto-prostaglandin F 1α(6-keto-PGF 1α) in human plasma, we obtained two antibodies which had apparently similar cross-reactivities with 22 other prostaglandins and related compounds. However, in radioimmunoassay, use of one of the antibodies gave results which were about double the results obtained with the other. The results obtained by both antibodies correlated significantly with each other according to the equation y = 0.43x − 13.0 pg/ml, R = 0.89, n = 58 (p < 0.001). The increments of plasma 6-keto-PGF 1α after an intravenous infusion of prostacyclin (PGI 2) also correlated with the dose of PGI 2 (R = 0.93 and 0.96, n = 24, p < 0.001) in both cases, and the increments obtained by the use of both antibodies correlated with each other (R = 0.94, n = 24, p < 0.001). The increments were, however, of different magnitude. These differences can be best explained by assuming different immunoreactivity of the two antibodies with metabolites of PGI2 other than 6-keto-PGF 1α, or with the two known stereochemical configuration of 6-keto-PGF 1α.
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