Abstract
Different strategies for simultaneous saccharification and fermentation (SSF) process were developed to enhance the productivity of ethanol by Kluyveromyces marxianus ATCC36907 as a part of biorefinery concept using cashew apple bagasse (CAB) as biomass. CAB was pretreated with 4.3% (v/v) alkaline hydrogen peroxide at pH 11.5 (CAB-AHP). Batch SSF conducted at 45 °C using 10% (w/v) CAB-AHP reported the highest concentration and ethanol yield. In the fed-batch SSF, the highest production (7.91 g ethanol/100gCAB) and ethanol yield (84.69%) were achieved using 10% (w/v) CAB-AHP initial solids load, 4% solid feeding at 24 h, and the feeding of enzymes only in the beginning of the process. The recycling of pretreatment inputs decreased 50% of the batch-SSF efficiencies, saving the consumption of water, H2O2, and NaOH in 66.67%, 48.44%, and 66.67%, respectively. Lignin was extracted from the hydrolysate with yield of 39.2%. Thus, the proposed biorefinery concept utilizes CAB to produce ethanol with high efficiency by fed-batch SSF, pretreatment input recycling, and the utilization of the extracted lignin for future applications.
Published Version
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