Abstract
BackgroundAlthough lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability in litter size of Xiang pig, we performed transcriptome analysis for the expression profile in ovaries using RNA-seq method.ResultsWe identified 1,419 up-regulated and 1,376 down-regulated genes in Xiang pigs with large litter size. Among them, 1,010 differentially expressed genes (DEGs) were differently spliced between two groups with large or small litter sizes. Based on GO and KEGG analysis, numerous members of genes were gathered in ovarian steroidogenesis, steroid biosynthesis, oocyte maturation and reproduction processes.ConclusionsCombined with gene biological function, twelve genes were found out that might be related with the reproductive capability of Xiang pig, of which, eleven genes were recognized as hub genes. These genes may play a role in promoting litter size by elevating steroid and peptide hormones supply through the ovary and facilitating the processes of ovulation and in vivo fertilization.
Highlights
Lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds
Samples A total of 40 Congjiang Xiang pigs were prepared from the farm of Dachang pig breeding, Congjiang, Guizhou, China, which born from sows ever giving birth of large litter size as XL group with the total number born (TNB) larger than twelve, or XS group with TNB less than eight
According to the reported functions in any similar paths that relate to ovarian steroidogenesis, fecundity, pregnant or embryo development, we identified 12 candidate genes (StAR, dehydrogenase/reductase SDR family member 4 (DHRS4), RLN2, PTX3, HSD3B, MSMO1, SCARB1, COX1, COX3, SELENOP, CYP11A1, and NR4A1) having a linkage with pig reproduction capability and litter size
Summary
Lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability in litter size of Xiang pig, we performed transcriptome analysis for the expression profile in ovaries using RNA-seq method. Reproductive traits are extremely intricate and influenced by multifactors originating from heredity and environment especially in litter size of pigs [2,3,4,5]. The different expression profiles of some important genes in ovary might devote to comprehend the diversity of litter size among breeds [8, 9]
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