Differences in bacterial community composition, structure and function between sediments in waterways and non-navigable channels in a plain river network area.

Abstract

Bacterial communities greatly help maintain the balance of river ecosystems and are highly sensitive to changes in environmental conditions. Plain river network areas (PRNs) are characterized by dense river networks, low-lying terrain, and slow water flow, where the bottom sediment is frequently disturbed by ship navigation due to the limited water depth and width of waterways, providing a unique ecological niche for bacterial growth. Hence, understanding how bacterial communities in PRNs respond to changes in hydrodynamic conditions, physicochemical parameters, and pollutants under ship navigation is essential to maintaining the stability of inland waterway ecosystems. The Taihu Lake Basin, a typical PRN, was selected to explore the differences in bacterial community composition, structure and function between sediments in waterways (WS) and non-navigable channels (NS). The results indicate that the sediment from NS possessed more diverse and complex bacterial communities than WS. NMDS and ANOSIM analyses further verified the significant differences in bacterial community structure between WS and NS. Combined with LEfSe, we observed the highly differential taxonomy between WS and NS from phylum to order. Moreover, a comparison of beta diversity dissimilarity indices revealed that although species replacement dominated both the WS and NS beta-diversity patterns, species loss caused the differences in the overall beta diversity between them. Variance partitioning analysis revealed that physicochemical parameters (clay content, pH, ORP, and others) and ship traffic volume (STV) were the main driving factors for bacterial community distribution between WS and NS, while pollutants (heavy metals, perfluoroalkyl acids, and others) had a relatively minor influence. PICRUSt2 analysis revealed that the changes in pH, ORP, and STV under ship navigation might inhibit the bacterial ability to metabolize carbohydrates. The results reveal the comprehensive effects of ship navigation disturbance on sediment bacterial communities in the PRN and contribute to further understanding of inland waterway ecosystems.