Abstract
As part of ongoing studies to characterize responses to controlled hemorrhage (Klemcke et al., Shock, in press, 2007), we conducted hematological and coagulation evaluations of 15 different inbred rat strains (n = 8–11 rats/strain). Rats were catheterized and, 24 hours later, 55% of their blood volume was removed. Complete blood counts (CBC), thromboelastographic (TEG) measures, plasma fibrinogen concentrations and prothrombin times (PT) were measured in samples taken during the initial phase of hemorrhage. Two rat strains tended to be the most divergent for many of these measures: Brown Norway/Medical College of Wisconsin (BN/Mcwi), and Munich Wistar Fromter (MWF). BN/Mcwi rats had greater PT (10.8 ± 0.2 vs 9.4 ± 0.1 sec), RBC (7.5 ± 0.1 vs 5.4 ± 0.2 106/mm3) and WBC (12.6 ± 0.4 vs 1.4 ± 0.1 103/mm3), but less fibrinogen (277 ± 21 vs 412 ± 13 mg/dl), platelets (408 ± 21 vs 776 ± 47 103/mm3), and lower clot strength based on TEG measures (64.7 ± 0.9 vs 75.9 ± 0.7 mm) than did Munich Wistar Fromter (MWF) rats (P <0.05). Hence, MWF rats appeared to exceed BN/Mcwi rats in many aspects of coagulation. Should the extremely low WBC counts of the MWF be shown to reflect an altered immune system function, these data suggest a potential utility for BN/Mcwi and MWF rats as models for investigation of the important interactions between coagulation and immunity (Am J Transplant 7:499, 2007).
Published Version
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