Abstract
When rat and Japanese quail hypothalamic extracts (HE) were prepared by acid or acid-ethanol extraction, LH-releasing activity of quail HE on rat pituitary glands was very low as compared with that of rat HE, both in vitro and in vivo. However, quail HE was higher in activity than was rat HE when tested on quail pituitary glands in vitro. The inhibition curve of rat HE was parallel to that of synthetic LH-RH in a radioimmunoassay system with the antisynthetic LH-RH serum, while quail HE did not show any significant cross-reaction. The antisynthetic LH-RH serum did not inhibit the biological activity of quail HE in vitro. These observations indicated a species-specificity among these LH-RFs. When quail hypothalami were extracted with neutral phosphate-buffered saline, a cross-reactive substance was found, though the inhibition curve was not parallel to that of synthetic LH-RH. Immunohistochemical observation using the same antisynthetic LH-RH serum and peroxidase-labeled anti-rabbit γ-globulin serum indicated the presence of immunoreactive substance(s) in the quail hypothalamus. The relationship between the immunoreactive substance(s) and cross-reaction in radioimmunoassay is also discussed.
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