Abstract
Objective: To compare the effect of rubbing and soaking tooth extraction instrument in 70%  alcohol on total oral bacterial colonisation at the Department of Oral Surgery, Faculty of Dentistry, Universitas Sumatera Utara. Material and Methods: This is an experimental research with a post-test only approach. The sampling technique used is Purposive Sampling. In this study, sample were divided into 2 groups consisting of 18 extraction instruments which are lower molar forceps rubbed with 70% alcohol and soaked in 70% alcohol. Each instrument were pre-cleaned using brush, water and soap prior disinfection process.Results: The results were statistically analyzed using Mann-Whitney Test. The result showed no significant difference to the total bacteria count on rubbing and soaking using 70% alcohol.Conclusion: Soaking in 70% alcohol method showed more effective because it overally kills bacteria.Keyword:Â
Highlights
Infection has been known as a real threat in the dental field
Forceps were removed from disinfectant and dried with sterile gauze and the beak of forceps were afterwards immersed in 50 ml of saline for 5 minutes and the container was closed tightly and sent to the microbiology laboratory for bacterial cultivation and colony bacteria count
The results showed that there were differences in the number of bacterial colonization in the 70% alcohol rubbing group and 70% soaking in alcohol group
Summary
Infection has been known as a real threat in the dental field. Tooth extraction is one form of dental treatment which has a high risk of transmission of infection. Disinfection using chemicals material can kill all bacteria and fungi and some viruses not spores.[3]. Disinfectant are divided into three groups, namely high level disinfectant such as glutaraldehyde, intermediate level disinfectant such as alcohol and low level disinfectant such as chlorine. Alcohol 70% is classified as an intermediate level disinfectant, where it can destroy vegetative bacteria, some fungi and viruses but not spores. Alcohol reacts by denaturing proteins and dissolving fat, as a result cell membranes are damaged and enzymes activated by alcohol.[4,5] Alcohol is bactericidal against vegetative cells, tuberculocidal, but not effective against hydrophilic viruses.[6]
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