Abstract
In order to identify the molecular size of receptors for α-rat atrial natriuretic polypeptide (α-rANP), we utilized the direct UV irradiation method for photoaffinity labeling with the biologically active [ 125I]α-rANP. In the preparation of isolated glomerulus and the inner medullary collecting duct (IMCT)-rich fraction, the autoradiograms of the electrophoresed sodium dodecyl sulfate (SDS)-polyacrylamide gels showed a single radioactive band which is displaceable with unlabeled α-rANP. The dose-dependent displacement fit very well with a binding-inhibition curve representing the binding affinity of 6.5×10 −10M. The molecular size of the ligand-receptor complex was about 65,000 daltons for both glomerulus and IMCT-rich fraction. In contrast, in homogenate of the aorta and adrenal gland, the ligand-receptor complex was 140,000 daltons.
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