Diethylaminoethyl Cellulose Immobilized Pointed Gourd (Trichosanthes dioica) Peroxidase in Decolorization of Synthetic Dyes

Abstract

Diethylaminoethyl (DEAE) cellulose adsorbed Pointed Gourd Peroxidase (PGP) was employed in decolorization of synthetic dyes. The expressed activity of immobilized preparation on fifth repeated use was ~50% and decolorization achieved for synthetic dyes DR19 and dye mixture (DR19+DB9) was 64.9% and 61.5% respectively. Immobilized enzyme could effectively decolorize up to 88.2% and 77.4% of DR19 and dye mixture respectively in stirred batch process at 40°C whereas dye color removal monitored at 30°C and 50°C was comparatively low under similar conditions. Immobilized enzyme in the packed column used for the continuous removal of dye color could successfully decolorize DR19 and dye mixture to 69.4% and 51.4% after 50 d of operation. Thus, DEAE immobilized PGP is a simple, economical and effective preparation to remove color of synthetic dyes.